In this paper, the influence of the bike frame on the loading of the eBike drive unit is determined. For this purpose, relevant load cases and boundary conditions are derived based on the existing norms for the individual consideration of the frame and the drive. In this context, external loads on the frame and the forces acting on the engine must be taken into account. The following simulative study shows that the bike frame has an enormous and load-dependent influence on the load situation of the frame interface and the stresses within the housing of the drive unit. In general, this influence can be explained by the stiffness of the frame construction. Altogether, these results show that the design of the eBike drive unit according to its current standard requirements is not sufficient to cover these enormous influences of the bike frame