A synthetic system to study the establishment of meiotic interhomolog-specific DNA repair by the Red1-Hop1-Mek1 complex

Meiosis is a specialised type of resulting in formation of gametes from diploid progenitor cells. In early meiotic prophase of budding yeast meiosis, introduction of DNA double stranded breaks occur in a programmed manner that are subsequently repaired by homologous recombination mechanism by using the homologous chromosome as a repair template. Typically in vegetatively dividing cells, a sister-chromatid is used as a template of choice for repairing DSBs. The biased use of homologous chromosome is brought upon by the action of meiosis specific factors – the Red1-Hop1-Mek1 complex (RHM complex). Here In this work, I describe the mechanism by which the RHM complex works in mediating the homolog bias. I design a synthetic system whereby I express components of this complex in mitotically dividing cells and monitor the activation and function of this complex. Our results indicate that the Mek1 kinase is active and functional and at least is able to mediate the homolog bias in parts.

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