000K  utf8
1100  2024$c2024-07-23
1500  eng
2050  urn:nbn:de:hbz:465-20240723-123838-1
2051  10.17185/duepublico/76868
3000  Nivsarkar, Vaishnavi Niraj
4000  synthetic system to study the establishment of meiotic interhomolog-specific DNA repair by the Red1-Hop1-Mek1 complex  [Nivsarkar, Vaishnavi Niraj]
4060  157, xiv Seiten
4209  Meiosis is a specialised type of resulting in formation of gametes from diploid progenitor cells. In early meiotic prophase of budding yeast meiosis, introduction of DNA double stranded breaks occur in a programmed manner that are subsequently repaired by homologous recombination mechanism by using the homologous chromosome as a repair template. Typically in vegetatively dividing cells, a sister-chromatid is used as a template of choice for repairing DSBs. The biased use of homologous chromosome is brought upon by the action of meiosis specific factors – the Red1-Hop1-Mek1 complex (RHM complex). Here In this work, I describe the mechanism by which the RHM complex works in mediating the homolog bias. I design a synthetic system whereby I express components of this complex in mitotically dividing cells and monitor the activation and function of this complex. Our results indicate that the Mek1 kinase is active and functional and at least is able to mediate the homolog bias in parts.
4950  https://doi.org/10.17185/duepublico/76868$xR$3Volltext$534
4950  https://nbn-resolving.org/urn:nbn:de:hbz:465-20240723-123838-1$xR$3Volltext$534
4961  https://duepublico2.uni-due.de/receive/duepublico_mods_00076868
5051  570
5550  budding yeast
5550  cell cycle
5550  DNA repair
5550  Meiose
5550  recombination
5550  Yeast genetics