Public transportation systems, including trams and buses, play a crucial role in urban traffic. However, these traditional modes of transport have some well-known drawbacks, such as long distances between stops, lengthy waiting times, and a lack of privacy. In response to these challenges, an innovative mobility concept called “FLAIT-train” offers potential solutions. The FLAIT-train operates on regular roads and aims to provide DOOR-2-DOOR transport, addressing the issues associated with fixed stops and offering increased accessibility and convenience. In its initial phase, the FLAIT-train operates on exclusive lanes, but it is designed to integrate with other traffic eventually. The vehicle technology of FLAIT-trains closely resembles that of battery electric autonomous vehicles. To assess whether FLAIT-trains can be used as a suitable alternative to conventional public transportation systems, this paper employs traffic simulations that consider key performance indicators, including the average waiting time per passenger, maximum waiting time of a single passenger, average in-vehicle time per passenger, and average occupancy rate of the vehicles. Using SUMO software (“Simulation of Urban Mobility”, version 1.12.0), a night bus service scenario is meticulously designed and generated. Within this scenario, both FLAIT-trains and conventional buses are simulated under identical conditions and based on statistical data.