Hepatitis B virus particles activate B cells through the TLR2–MyD88–mTOR axis

Li, Qian; Wang, Jun; Islam, Heba; Kirschning, Carsten; Lu, Hongzhou; Hoffmann, Daniel; Dittmer, Ulf; Lu, Mengji

doi:10.1038/s41419-020-03284-1

Artikel / Aufsatz Mo., 04. Jan.. 2021 CC BY 4.0

Veröffentlicht

Hepatitis B virus particles activate B cells through the TLR2–MyD88–mTOR axis

Li, Qian ; Wang, Jun ; Islam, Heba ; Kirschning, Carsten ; Lu, Hongzhou ; Hoffmann, Daniel ; Dittmer, Ulf ; Lu, Mengji

Host immune control plays a pivotal role in resolving primary hepatitis-B-virus (HBV) infections. The complex interaction between HBV and host immune cells, however, remains unclear. In this study, the transcriptional profiling of specimens from animals infected with woodchuck hepatitis virus (WHV) indicated TLR2 mRNA accumulation as most strongly impacted during WHV infection resolution as compared to other mRNAs. Analysis of blood transcriptional modules demonstrated that monocytes and B-cells were the predominantly activated cell types in animals that showed resolution of infection, which was similar to the response of TLR2-stimulated PBMCs. Further investigation of TLR2-stimulated B-cells pointed at interactions between activated TLR signaling, Akt-mTOR, and glucose metabolic pathways. Moreover, analysis of B-cells from Tlr2^−/−, Trif^−/− , Myd88^−/− , and Trif/Myd88^−/− mice challenged with HBV particles indicated B-cell function and glucose metabolism alterations is TLR2-MyD88-mTOR axis dependent. Overall, our study implicates B-cell TLR2 activation in HBV infection resolution.

Vorschau

Einordnung

Datum der Veröffentlichung:

04.01.2021

URN:

urn:nbn:de:hbz:465-20240828-091927-7

DOI:

10.1038/s41419-020-03284-1

Sprache:

Englisch

Ressourcentyp:

Text

Schlagwörter:

Pattern recognition receptors; Viral infection

Kollektion:

E-Publikationen

Sachgruppen der Deutschen Nationalbibliographie:

610 Medizin, Gesundheit

Sachgruppen der Deutschen Nationalbibliographie:

570 Biowissenschaften, Biologie

Einrichtung:

Medizinische Fakultät, Universitätsklinikum Essen, Institut für Virologie

Einrichtung:

Medizinische Fakultät, Universitätsklinikum Essen, Institut für Medizinische Mikrobiologie

Einrichtung:

Fakultät für Biologie, Bioinformatics and Computational Biophysics

Einrichtung:

Forschungszentren, Zentrum für Medizinische Biotechnologie (ZMB)

Förderung:

The publication of this article was supported by the Publication Fund of the University of Duisburg-Essen.

The authors are grateful for the support from Deutsche Forschungsgemeinschaft (TRR60 and RTG 1949/2) and the National Natural Science Foundation of China for funding the work of this study. M.L. was supported by grants from the Deutsche Forschungsgemeinschaft (RTG1949/1 and Transregio TRR60). J.W. was supported by the foundation of Wuxi Young Medical Talents, Health Bureau of Wuxi (MS201731, Q201743, and CSE31N1712), and Wuxi Medical Development Discipline (FZXK006).

Open Access funding enabled and organized by Projekt DEAL.

Informationen zur Erstveröffentlichung:

Li, Q., Wang, J., Islam, H. et al. Hepatitis B virus particles activate B cells through the TLR2–MyD88–mTOR axis. Cell Death Dis 12, 34 (2021). https://doi.org/10.1038/s41419-020-03284-1

Published 04 January 2021

Versionskennzeichen: