Intelligent vehicles gradually enter the vehicular fleet with advanced driver-assistance technologies. Their impact on traffic should, therefore, be considered by transportation decision-makers. This paper examines the effect of vehicles with different levels of automation on traffic flow, such as non-assisted vehicles, vehicles with driver assistance systems, and fully autonomous vehicles. The accuracy of the examined traffic scenario is also an important factor in microscopic traffic simulation. In this paper, the central part of the city of Duisburg, Duisburg’s inner ring, is chosen for the traffic scenario. Through the cooperation with local government, official data of Origin/Destination matrices, induction loops, and traffic light plans are provided for this work. Thus, traffic demand from Origin/Destination matrices and induction loops are generated and compared, respectively. Finally, vehicles with different levels of automation are simulated in the Duisburg inner ring scenario.