This paper studies the relationship between cultural values and gender distribution across fields of study in higher education. I compute national, field and subfield-level gender segregation indices for a panel dataset of 26 OECD countries for 1998–2012. This panel dataset expands the focus of previous macro-level research by exploiting data on gender segregation in specific subfields of study. Fixed-effects estimates associate higher country-level religiosity with lower gender segregation in higher education. These models crucially control for potential segregation factors, such as labor market and educational institutions, and gender gaps in both self-beliefs and academic performance in math among young people.