Generation of two H1 hESC sublines carrying deletions of RB1 exon 1/promoter in heterozygous or compound heterozygous state

Menges, Julia; Cremanns, Martina; Steenpass, Laura

doi:10.1016/j.scr.2019.101517

Article / Chapter Mon Jul 29 2019 CC BY-NC-ND 4.0

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Generation of two H1 hESC sublines carrying deletions of RB1 exon 1/promoter in heterozygous or compound heterozygous state

Menges, Julia; Cremanns, Martina ^GND; Steenpass, Laura

Biallelic inactivation of the retinoblastoma tumor suppressor gene (RB1) causes formation of retinoblastoma, a retinal eye tumor occurring in early childhood. Using the CRISPR/Cas9 nickase system, exon 1 of RB1 was deleted, including the RB1 promoter. As a result, sublines were generated carrying deletions of RB1 exon 1/promoter on one or both alleles.

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Menges, Julia / Cremanns, Martina / Steenpass, Laura: Generation of two H1 hESC sublines carrying deletions of RB1 exon 1/promoter in heterozygous or compound heterozygous state. 2019.

10.1016/j.scr.2019.101517
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