Generation of two H1 hESC sublines carrying deletions of RB1 exon 1/promoter in heterozygous or compound heterozygous state
Biallelic inactivation of the retinoblastoma tumor suppressor gene (RB1) causes formation of retinoblastoma, a retinal eye tumor occurring in early childhood. Using the CRISPR/Cas9 nickase system, exon 1 of RB1 was deleted, including the RB1 promoter. As a result, sublines were generated carrying deletions of RB1 exon 1/promoter on one or both alleles.
Preview
Cite
Citation style:
Menges, J., Cremanns, M., Steenpass, L., 2019. Generation of two H1 hESC sublines carrying deletions of RB1 exon 1/promoter in heterozygous or compound heterozygous state. https://doi.org/10.1016/j.scr.2019.101517
Could not load citation form.
Rights
Use and reproduction:
This work may be used under a
Creative Commons Attribution - NonCommercial - NoDerivatives 4.0 License (CC BY-NC-ND 4.0)
.