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Generation of two H1 hESC sublines carrying deletions of RB1 exon 1/promoter in heterozygous or compound heterozygous state

Biallelic inactivation of the retinoblastoma tumor suppressor gene (RB1) causes formation of retinoblastoma, a retinal eye tumor occurring in early childhood. Using the CRISPR/Cas9 nickase system, exon 1 of RB1 was deleted, including the RB1 promoter. As a result, sublines were generated carrying deletions of RB1 exon 1/promoter on one or both alleles.

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Date Issued:
29.07.2019
URN:
urn:nbn:de:hbz:464-20190813-162405-0
DOI:
10.1016/j.scr.2019.101517
Language:
English
Type of Resource:
Text
Collection:
E-Publications
Dewey Decimal Classification:
610 Medicine & health
Subject categories of the Deutsche Nationalbibliografie:
610 Medical sciences Medicine
Hosting institution:
Faculty of Medicine, Essen University Hospital, Institute of Human Genetics
Hosting institution:
Faculty of Medicine, Essen University Hospital, Institute of Transfusion Medicine
Info on primary publication:
Stem Cell Research 39 (2019) 101517; Available online 29 July 2019
funding:

The publication of this article was supported by the Publication Fund of the University of Duisburg-Essen.

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