The ATP-binding cassette transporters ABCB1 and ABCC1 are not regulated by hypoxia in immortalised human brain microvascular endothelial cells
Methods: In order to investigate whether hypoxia might be responsible for the expression changes of ABCB1 and ABCC1 in the ischemic brain, the immortalised human brain microvascular endothelial cell line hCMEC/D3 was exposed to hypoxia (1%) or anoxia (0%). Cell lysates were analysed by Western blot to detect the protein expression of ABCB1, ABCC1, HIF-1a and HIF-2a.
Results: During hypoxia, an accumulation of HIF-1a and HIF-2a was noticed in hCMEC/D3 cells that followed different time kinetics. Both HIF-1a and HIF-2a abundance increased within 4 h of hypoxia. HIF-1a levels decreased to below detection levels within 16 h of hypoxia, whereas HIF-2a remained elevated even after 48 h. No changes of ABCB1 and ABCC1 expression were detected, neither on the mRNA nor protein level.
Conclusion: Our data suggests that other factors than hypoxia may be responsible for the expression changes of ATP-binding cassette transporters in the ischemic brain.
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