Analysis of drug sensitivity of human high-grade osteosarcoma in a chick chorioallantoic membrane (CAM) model: a proof of principle study

GND
109579230X
ORCID
0000-0001-9367-7130
Affiliation
Division of Musculoskeletal Oncology, University Hospital Essen, Essen, Germany
Guder, Wiebke K.;
GND
121932695X
ORCID
0000-0002-7609-5021
Affiliation
Gerhard Domagk Institute of Pathology, University Hospital Muenster, Münster, Germany
Hartmann, Wolfgang;
GND
1054566585
ORCID
0000-0002-5842-1196
Affiliation
Gerhard Domagk Institute of Pathology, University Hospital Muenster, Münster, Germany
Trautmann, Marcel;
GND
124136923
Affiliation
Division of Musculoskeletal Oncology, University Hospital Essen, Essen, Germany
Hardes, Jendrik;
GND
121391175
ORCID
0000-0001-6788-4910
Affiliation
Gerhard Domagk Institute of Pathology, University Hospital Muenster, Münster, Germany
Wardelmann, Eva;
GND
1060261308
ORCID
0000-0002-9699-370X
Affiliation
Sportsclinic Cologne, Cologne, Germany
Balke, Maurice;
GND
129565180
Affiliation
Division of Musculoskeletal Oncology, University Hospital Essen, Essen, Germany
Streitbürger, Arne

Objective: Multi-agent chemotherapy is an important pillar in treatment of high-grade osteosarcoma. In an effort to improve patient survival, it is imperative to determine the effectiveness of new substances. The objective of this study was to investigate whether the chick chorioallantoic membrane (CAM) model can be used to analyze drug sensitivity in high-grade osteosarcoma.

Results: Spare biopsy tissue from five patients diagnosed with high-grade osteosarcoma was transferred into non-immortalized primary cell culture. After a pre-incubation period of 10 days, fertilized chick eggs were inoculated with primary tumor cells suspended in extracellular matrix gel. On day 16, treatment with 20 µmol/l doxorubicin (n = 4) or 25 µl of culture medium (n = 6) was performed for 24 h. CAM membranes were documented macroscopically, harvested and examined histologically. Transfer of biopsy specimens into primary cell culture was successful in all cases. 50% (n = 10) of eggs died after inoculation with tumor cells and before application of doxorubicin. No deaths occurred after application of doxorubicin. Histological examination found a response to doxorubicin in all four specimens. Based upon these results, the CAM model represents a promising preclinical alternative to animal experiments to determine drug sensitivity of osteosarcoma cells. Further research with regard to other substances and dosages appear justified.

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