This paper proposes a novel single-layer, low-profile harmonic transponder to be integrated with the printable diodes based on Si particles. The introduced prototype consists of two bowtie dipoles that are directly matched to the harmonic generation element at a fundamental 2.45GHz frequency and also at the corresponding second harmonic 4.9GHz frequency. Therefore, the antennas and T-matching parts can be manufactured as separate inlays using a single layer-substrate. Besides the new prototype, the harmonic conversion loss (CL) is theoretically and experimentally investigated. In this regard, the equivalent circuit is driven and utilized to analyze the CL performance with variations in fundamental frequency and input power. The introduced transponder outperforms the state-of-the-art work from the printability, size, and CL point of views.