Biallelic and monoallelic deletion of the RB1 promoter in six isogenic clonal H9 hESC lines.

Döpper, Hannah GND; Horstmann, Marius; Menges, Julia; Bozet, Morgane; Kanber, Deniz GND; Steenpass, Laura GND

Retinoblastoma is a childhood tumor of the retina that is caused mostly by biallelic inactivation of the tumor suppressor gene RB1. To generate a research resource, we abrogated expression of RB1 in H9 hESCs by CRISPR/Cas9 induced deletion of the RB1 promoter, either on one or on both alleles. This enables studies on the role of RB1 loss during differentiation, for example in differentiation towards neural retina. The generation of three isogenic lines per deletion state enables validation of phenotypic results in independent clonal lines.

Cite

Citation style:
Döpper, H., Horstmann, M., Menges, J., Bozet, M., Kanber, D., Steenpass, L., 2020. Biallelic and monoallelic deletion of the RB1 promoter in six isogenic clonal H9 hESC lines. https://doi.org/10.1016/j.scr.2020.101779
Could not load citation form.

Rights

Export