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An efficient ELISA protocol for measurement of SARS-CoV-2 spike-specific IgG in human plasma and serum samples

Here, we describe a protocol for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike-specific immunoglobulin G (IgG) by enzyme-linked immunosorbent assay (ELISA). The protocol was developed with a keen focus on optimizing several key parameters, including antigen coating concentration, antibody and sample dilutions, and assay development time. The final protocol features the following characteristics:
  • The capability to detect SARS-CoV-2 spike-specific IgG in both plasma and serum samples.
  • A streamlined procedure that requires only 1 hour and 20 minutes of hands-on time.
  • Reliable assay performance, with a remarkable sensitivity of 98.1 % and specificity of 99.5 %.

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Date Issued:
15.02.2024
URN:
urn:nbn:de:hbz:465-20250107-124446-5
DOI:
10.1016/j.mex.2024.102596
Language:
English
Type of Resource:
Text
Keywords:
SARS-CoV-2 spike; ELISA; IgG
Collection:
E-Publications
Subject categories of the Deutsche Nationalbibliografie:
610 Medical sciences Medicine
Hosting institution:
Faculty of Medicine, Essen University Hospital, Institute for HIV Research
Hosting institution:
Faculty of Medicine, Essen University Hospital, Institute of Transfusion Medicine
funding:
The research described in this work received support from the Foundation University Medicine Essen through the Grant (2020 4699 119) awarded to CBK and by the Open Access Publication Fund of the University of Duisburg-Essen.
Info on primary publication:

Appeltrath, G. A., Parreuter, J., Lindemann, M., Klump, H., & Karsten, C. B.(2024)  An efficient ELISA protocol for measurement of SARS-CoV-2 spike-specific IgG in human plasma and serum samples. MethodsX 12:102596. https://doi.org/10.1016/j.mex.2024.102596

Available online 5 February 2024

version identification:
Verlagsversion / Version of Record

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