@Article{duepublico_mods_00077556,
  author = 	{Welzel, Marius
		and Lange, Anja
		and Heider, Dominik
		and Schwarz, Michael
		and Freisleben, Bernd
		and Jensen, Manfred
		and Boenigk, Jens
		and Beisser, Daniela},
  title = 	{Natrix: a Snakemake-based workflow for processing, clustering, and taxonomically assigning amplicon sequencing reads},
  year = 	{2020},
  month = 	{Nov},
  day = 	{16},
  keywords = 	{Amplicon sequencing; Operational Taxonomic Units; Amplicon Sequence Variants; Snakemake; Pipline; Illumina},
  abstract = 	{Background: Sequencing of marker genes amplified from environmental samples, known as amplicon sequencing, allows us to resolve some of the hidden diversity and elucidate evolutionary relationships and ecological processes among complex microbial communities. The analysis of large numbers of samples at high sequencing depths generated by high throughput sequencing technologies requires efficient, flexible, and reproducible bioinformatics pipelines. Only a few existing workflows can be run in a user-friendly, scalable, and reproducible manner on different computing devices using an efficient workflow management system. Results: We present Natrix, an open-source bioinformatics workflow for preprocessing raw amplicon sequencing data. The workflow contains all analysis steps from quality assessment, read assembly, dereplication, chimera detection, split-sample merging, sequence representative assignment (OTUs or ASVs) to the taxonomic assignment of sequence representatives. The workflow is written using Snakemake, a workflow management engine for developing data analysis workflows. In addition, Conda is used for version control. Thus, Snakemake ensures reproducibility and Conda offers version control of the utilized programs. The encapsulation of rules and their dependencies support hassle-free sharing of rules between workflows and easy adaptation and extension of existing workflows. Natrix is freely available on GitHub ( https://github.com/MW55/Natrix ) or as a Docker container on DockerHub ( https://hub.docker.com/r/mw55/natrix ). Conclusion: Natrix is a user-friendly and highly extensible workflow for processing Illumina amplicon data.},
  note = 	{<p>The publication of this article was supported by the Publication Fund of the University of Duisburg-Essen.</p>},
  note = 	{<p>Welzel, M., Lange, A., Heider, D. <em>et al.</em> Natrix: a Snakemake-based workflow for processing, clustering, and taxonomically assigning amplicon sequencing reads. <em>BMC Bioinformatics</em> <strong> 21</strong>, 526 (2020). <a href="https://doi.org/10.1186/s12859-020-03852-4">https://doi.org/10.1186/s12859-020-03852-4</a></p>

<p>Published: 16 November 2020</p>},
  note = 	{Version of Record / Verlagsversion},
  doi = 	{10.1186/s12859-020-03852-4},
  url = 	{https://duepublico2.uni-due.de/receive/duepublico_mods_00077556},
  url = 	{https://doi.org/10.1186/s12859-020-03852-4},
  file = 	{:https://duepublico2.uni-due.de/servlets/MCRFileNodeServlet/duepublico_derivate_00077207/BMC_Bioinformatics_2020_21_526.pdf:PDF},
  language = 	{en}
}