000K utf8 1100 2022$c2022-09-13 1500 eng 2050 urn:nbn:de:hbz:465-20220922-144614-1 2051 10.3390/v14092031 3000 Sperber, Hannah Sabeth 3010 Müller, Karin 3010 Müller, Peter 3010 Schwarzer, Roland 3010 Sutter, Kathrin 4000 Bovine Seminal Plasma Protein PDC-109 Possesses Pan-Antiviral Activity [Sperber, Hannah Sabeth] 4209 Mammalian seminal plasma contains a multitude of bioactive components, including lipids, glucose, mineral elements, metabolites, proteins, cytokines, and growth factors, with various functions during insemination and fertilization. The seminal plasma protein PDC-109 is one of the major soluble components of the bovine ejaculate and is crucially important for sperm motility, capacitation, and acrosome reaction. A hitherto underappreciated function of seminal plasma is its anti-microbial and antiviral activity, which may limit the sexual transmission of infectious diseases during intercourse. We have recently discovered that PDC-109 inhibits the membrane fusion activity of influenza virus particles and significantly impairs viral infections at micromolar concentrations. Here we investigated whether the antiviral activity of PDC-109 is restricted to Influenza or if other mammalian viruses are similarly affected. We focused on Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), the etiological agent of the Coronavirus Disease 19 (COVID-19), thoroughly assessing PDC-109 inhibition with SARS-CoV-2 Spike (S)-pseudotyped reporter virus particles, but also live-virus infections. Consistent with our previous publications, we found significant virus inhibition, albeit accompanied by substantial cytotoxicity. However, using time-of-addition experiments we discovered a treatment regimen that enables virus suppression without affecting cell viability. We furthermore demonstrated that PDC-109 is also able to impair infections mediated by the VSV glycoprotein (VSVg), thus indicating a broad pan-antiviral activity against multiple virus species and families. 4950 https://doi.org/10.3390/v14092031$xR$3Volltext$534 4950 https://nbn-resolving.org/urn:nbn:de:hbz:465-20220922-144614-1$xR$3Volltext$534 4961 https://duepublico2.uni-due.de/receive/duepublico_mods_00076932 5051 610 5550 Bovine seminal plasma 5550 Fn-type 2 proteins 5550 PDC-109 5550 SARS-CoV-2 5550 VSV replicon