000K utf8 1100 2019$c2019-10-07 1500 eng 2050 urn:nbn:de:hbz:464-20191127-140742-7 2051 10.3390/cancers11101499 3000 Dünker, Nicole 3010 Jendrossek, Verena 4000 Implementation of the Chick Chorioallantoic Membrane (CAM) Model in Radiation Biology and Experimental Radiation Oncology Research. [Dünker, Nicole] 4209 Radiotherapy (RT) is part of standard cancer treatment. Innovations in treatment planning and increased precision in dose delivery have significantly improved the therapeutic gain of radiotherapy but are reaching their limits due to biologic constraints. Thus, a better understanding of the complex local and systemic responses to RT and of the biological mechanisms causing treatment success or failure is required if we aim to define novel targets for biological therapy optimization. Moreover, optimal treatment schedules and prognostic biomarkers have to be defined for assigning patients to the best treatment option. The complexity of the tumor environment and of the radiation response requires extensive in vivo experiments for the validation of such treatments. So far in vivo investigations have mostly been performed in time- and cost-intensive murine models. Here we propose the implementation of the chick chorioallantoic membrane (CAM) model as a fast, cost-efficient model for semi high-throughput preclinical in vivo screening of the modulation of the radiation effects by molecularly targeted drugs. This review provides a comprehensive overview on the application spectrum, advantages and limitations of the CAM assay and summarizes current knowledge of its applicability for cancer research with special focus on research in radiation biology and experimental radiation oncology. 4950 https://doi.org/10.3390/cancers11101499$xR$3Volltext$534 4950 https://nbn-resolving.org/urn:nbn:de:hbz:464-20191127-140742-7$xR$3Volltext$534 4961 https://duepublico2.uni-due.de/receive/duepublico_mods_00070807 5051 610 5550 CaM 5550 Cancer 5550 Chorioallantoic Membrane Assay 5550 Hypoxia 5550 Molecularly Targeted Drugs 5550 Radiation 5550 Radioresistance 5550 Radiotherapy 5550 Tumor 5550 Tumor Microenvironment