B-cell dynamics during experimental endotoxemia in humans

Brinkhoff, Alexandra LSF; Zeng, Ye; Sieberichs, Annette; Dolff, Sebastian LSF; Shilei, Xu; Sun, Ming; Engler, Harald LSF; Benson, Sven LSF; Korth, Johannes LSF; Schedlowski, Manfred LSF; Kribben, Andreas LSF; Witzke, Oliver LSF; Wilde, Benjamin LSF

Recently, B cells with regulatory functions suppressing T-cell immunity were identified. Inflammation in the context of sepsis is characterized by a profound immune dysfunction increasing the patient’s risk for additional infections. The impact of endotoxemia on B-cell dynamics, regulatory B cells (Breg) and its contribution to immune dysfunction is unknown. It is the aim of the present study to characterize the dynamics of the B-cell compartment and Breg in an experimental human endotoxemia model.
In this randomized placebo-controlled cross-over study, 20 healthy males received an intravenous injection of endotoxin (Escherichia coli lipopolysaccharide, LPS, 0.8 ng/kg body weight) or placebo (saline 0.9%) on two otherwise identical study days. B cells were analyzed by flow cytometry at baseline and repeatedly up to 72 h after endotoxin/placebo injection.
Absolute CD19⁺ B cells counts showed a significant decrease 3 h after endotoxin injection. Memory B cells were partially depleted from the circulation; the total number of Breg was significantly diminished 3 h after LPS challenge. Production of anti-inflammatory interleukin (IL)-10 (IL-10) by Breg was unaltered after LPS challenge. Systemic B-cell activating factor (BAFF) levelswere significantly increased with amaximum after 24 h and remained increased up to 72 h post-injection.

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Brinkhoff, Alexandra / Zeng, Ye / Sieberichs, Annette / et al: B-cell dynamics during experimental endotoxemia in humans. 2019.

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