000K  utf8
1100  2014$c2014-12-09
1500  eng
2050  urn:nbn:de:hbz:464-20160519-075233-9
2051  10.1111/1751-7915.12188
3000  Zhang, Ruiyong
3010  Bellenberg, Sören
3010  Kuhlicke, Ute
3010  Neu, Thomas R.
3010  Sand, Wolfgang
3010  Vera, Mario
4000  Use of lectins to in situ visualize glycoconjugates of extracellular polymeric substances in acidophilic archaeal biofilms  [Zhang, Ruiyong]
4209  Biofilm formation and the production of extracellular polymeric substances (EPS) by meso- and thermoacidophilic metal-oxidizing archaea on relevant substrates have been studied to a limited extent. In order to investigate glycoconjugates, a major part of the EPS, during biofilm formation/bioleaching by archaea on pyrite, a screening with 75 commercially available lectins by fluorescence lectin-binding analysis (FLBA) has been performed. Three representative archaeal species, Ferroplasma acidiphilum DSM 28986, Sulfolobus metallicus DSM 6482T and a novel isolate Acidianus sp. DSM 29099 were used. In addition, Acidianus sp. DSM 29099 biofilms on elemental sulfur were studied. The results of FLBA indicate (i) 22 lectins bound to archaeal biofilms on pyrite and 21 lectins were binding to Acidianus sp. DSM 29099 biofilms on elemental sulfur; (ii) major binding patterns, e.g. tightly bound EPS and loosely bound EPS, were detected on both substrates; (iii) the three archaeal species produced various EPS glycoconjugates on pyrite surfaces. Additionally, the substratum induced different EPS glycoconjugates and biofilm structures of cells of Acidianus sp. DSM 29099. Our data provide new insights into interactions between acidophilic archaea on relevant surfaces and also indicate that FLBA is a valuable tool for in situ investigations on archaeal biofilms.
4950  https://doi.org/10.1111/1751-7915.12188$xR$3Volltext$534
4950  https://nbn-resolving.org/urn:nbn:de:hbz:464-20160519-075233-9$xR$3Volltext$534
4961  https://duepublico2.uni-due.de/receive/duepublico_mods_00041299
5010  54
5051  540